PCR方法检测犬细小病毒生长动态及与其它方法的比较

Comparison among PCR, HA and ELISA for Detecting the Proliferation of CPV Growing in Some Kinds of Cell

将可疑犬细小病毒 ( CPV)粪样分别接种于 FK81、MDCK、BHK2 1、Vero细胞及鸡胚成纤维细胞中 ,用 PCR、HA、EL ISA三种方法检测在上面 5种细胞中 CPV的生长动态 ,结果表明 :PCR方法能在第 1、2、3代 FK81细胞中检出 CPV的生长 ,而 HA、EL ISA则只在第 3代 FK81细胞中才能检出 CPV的生长 ;PCR方法能在 MDCK、BHK2 1、Vero细胞及鸡胚成纤维细胞中检出 CPV的生长 ,而 HA、EL ISA则均不能。因此 ,PCR方法可作为培养细胞中检测病毒生长动态的一种灵敏、快速的方法。

CPV was inoculated in FK 81 ,MDCK,BHK 21 ,Vero cell and embryonated egg cell. The proliferation of CPV was detected with PCR, HA and ELISA. The result showed that the PCR is more sensitivity than HA and ELISA for detecting the proliferation of CPV. It was suggested that PCR should be a Choice for detecting the proliferation of CPV growing in some kind of cells.