新西兰兔髂动脉血管球囊导管损伤后内膜肥厚模型的建立

The Animal Model on Intimal Hyperplasia after Balloon Injury in Rabbits

目的 探讨兔髂动脉急性损伤后 ,血清中内皮素、血栓素A2 和前列环素水平的变化及血管内膜的改变。方法 12只新西兰大耳白兔 ,行右髂动脉球囊导管内膜剥脱术 ,术前、术后 3h及 3d放射免疫法测定血清中内皮素、血栓素A2 和前列环素浓度 ,两个月后取髂动脉测定血管内膜变化。结果 兔髂动脉损伤后血清内皮素即明显增加 ,血清中血栓素A2 浓度明显升高 ,前列环素水平则显著下降 ,血管内膜明显增厚 ,管腔狭窄 ,腔壁比 (wall lumen)显著低于对侧髂动脉。结论 髂动脉急性损伤后内皮素和血栓素A2 的升高、前列环素的水平降低及血管内膜明显增厚 。

Objective To investigate the alteration of serum endothelin,thromboxane A 2, prostacyclin and vascular intimal following injury by balloon in rabbit iliac artery.Methods The iliac arteries of twelve white New Zealand rabbits were injured with a inflated balloon catheter, and serum concentration of endothelin,thromboxane A 2 and prostacyclin was measured with radioimmunoassay before injury,3 hours and 3 days after balloon injury.Result Serum endothelin and thromboxane A 2 concentration significantly increased after injury,following injury by balloon,whereas serum prostacyclin markedly decreased.Vascular intimal markedly was hyperplasia in contrast to control. Conclusion Serum endothelin and thromboxane A 2 were increased,prostacyclin was decreased,which may result in thrombosis following attenuation of the vascular after the intimal hyperplasia by balloon injury.