植物组织培养中污染的鉴定与防止初步研究

PRELIMINARY STUDY ON THE CONTAMINATION IDENTIFYING AND PREVENTION IN PLANT TISSUE CULTURE

对学院植物组织培养实验室部分受污染的组培苗进行了污染物的分类与鉴定 ,发现棒杆菌属 (Corynebac terium)、肠杆菌属 (Enterobacter)、葡萄球菌属 (Staphylococus)、地霉属 (Geotrichum)、曲霉属 (Aspergillus)、毛霉属(Mucor)、根霉属 (Rhizopus)等常见的细菌和真菌是引起组培苗严重污染的主要菌类。取样测试鉴定表明 ,大部分的微生物来源于无菌接种室内的空气、超净工作台鼓风机排出的气体。抑菌试验发现 ,2mg/L的庆大霉素和 0 .3%质量分数的丙酸钠对细菌均有明显的抑制作用。

The identifying of the contaminating tissue culture seedling were tested. It was indicated that Corynebacterium, Enterobacter, Staphylococus, Geotrichum, Mucor, Aspergillus, Rhizopus and other genera were the main microbes bringing on contamination of tissue culture. Most of these microbes were originated from the indoor air of inoculating room, air-blower orifice and margin of the inoculating table. It was also tested that both 2mg/l gentamicin and 0.3% sodium propionate had obvious antibiotic effective. \;