蛋白激酶C同工酶在糖尿病肾病肾小球的表达

Expression of protein kinase C isoforms in glomerular cells of diabetic rats

目的 探讨糖尿病肾病（DN）发病过程中肾小球蛋白激酶C（PKC）同工酶的表达变化及其与DN发生、发展的关系。方法 将实验动物随机分为正常对照组及STZ-DM模型组。在模型成功后2周、4周和12周,采用免疫组织化学方法检测肾小球PKC同工酶的表达变化情况。结果 在肾小球内,PKCα在DM发病2周、4周、12周时表达显著上升;PKCβⅠ、PKCβⅡ在DM发病2周时表达明显下降,4周、12周时逐渐上升;PKCε的表达无显著性变化。结论 在DN发病过程中的不同阶段,PKC各同工酶在肾小球内的表达变化不同,对DN的发生、发展也有着不同的调控效应。

Objective To investigate the expression of protein kinase Ca,B1,B2,s isoforms in glomerular cells of diabetic rats and evaluate the relation between the altered expression of PKC isoforms and the pathogenesis of diabetic nephropathy. Methods SD rats were randomly divided into control group and diabetic group. Diabetic models were cofirmed by blood glucose>5 13. 9 mmol/L two days after STZ( 60 mg/kg) administration. Age-matched normal rats received 3 ml/kg bw 0. 9% saline as control group. The expression of PKC isoforms in control and diabetic glomerular cells was assessed by immunohistochemistry at 2 weeks, 4 weeks and 12 weeks after the onset of hyperglycemia. Results Immunohistochemistry revealed a significant increase of the expression of PKCa in glomeruli cells at 2 weeks, 4 weeks and 12 weeks. A significant decrease of PKCB1,B2 in glomerular cells at 2 weeks and a gradually increase at 4 weeks and 12 weeks were found; No significant change of the expression of PKC e in the diabetic glomerular cells was observed. Conclusions The expression of PKCa, B1,B2 e isoforms varies in different stages of diabetic nephropathy. The effect of PKC isoforms on the pathogenesis of diabetic nephropathy is different.