肿瘤细胞的进行性增殖和Bip/GRP78的合成

Progressing growth of tumor cell and synthesis of Bip/GRP78

目的 探讨肿瘤细胞生长和Bip/GRP78合成的关系。方法 利用体外肿瘤细胞培养 ,离子交换色谱、SDS PAGE、特异性酶、化学降解和质谱分析等手段 ,检查生长在指数生长期、汇合期及汇合后期MCF 7、MDA MB 2 31两种人乳腺癌细胞Bip/GRP78的合成情况 ,并与正常人乳腺上皮细胞进行比较。结果 在两种肿瘤细胞进行性生长增殖过程中 ,Bip/GRP78呈赖生长状态、赖细胞密度和赖恶性程度性暴发性合成。结论 肿瘤细胞在生长增殖过程中 ,能通过赖生长状态、赖细胞密度和赖恶性程度性地合成Bip/GRP78来维持其内环境的稳定 ,构筑自身防御机制。因为大量的研究业已证实Bip/GRP78能降低细胞毒性T细胞对瘤细胞的杀伤力、促成肿瘤的生成和抗药性产生、防止肿瘤细胞凋亡等 ,因此有针对性地破坏肿瘤细胞Bip/GRP78合成 ,可为肿瘤的治疗提供一个新方法。

AIM To explore the relationship between tumor progressing growth and synthesis of Bip/GRP78 in vitro. METHOD Using tumor cell culture, ion exchange chromatography, SDS-PAGE, specific enzymatic , chemical catalysis, mass spectra and so on, the synthesis of Bip/GRP78 of cells growth in exponential, confluent and post-confluent phases was examined,and compared to normal breast epithelial cells. RESULTS During the progressing growth, tumor cells' synthesis of Bip/GRP78 exhibited growth situation, cell density and malignant degree-dependent. CONCLUSIONS During the progressing growth, tumor cells can maintain its homeostasis by synthesizing Bip/GRP78. This synthesis is intensely growth situation. Cell density and malignant degree-dependent. By this synthesis, tumor cell establishs its defensive system. Because increasing investigate results have shown that Bip/GRP78 can decrease the sensitivity of tumor cell to be killed by cytotoxic T lymphocytes, increase its tumorigencity and prevent its apoptosis. So aiming at destruction of the synthesis of Bip/GRP78 may point to a new approaches to the therapy of cancer.