梨孢镰刀菌培养物提取后残渣对大鼠前胃致瘤作用的研究

Tumorigenesis of rat forestomach induced by residual of Fusarium Poae culture

本研究用梨孢镰刀菌培养物经70％乙醇、乙醚及生理盐水提取后的残渣,混以玉米面饲喂大鼠。对照组喂单纯玉米面,实验432天。实验组67.6％的动物前胃肉眼上出现粘膜粗糙、圆盘状病变或乳头状瘤。光镜下有73.5％的动物前胃出现癌前病变。对照组动物前胃均无明显变化。说明培养物虽经多次提取,其残渣仍有致瘤作用。

After the extraction of Fusarium Poae culture with 70% alcohol, ether andnormal saline, the residual was mixed in corn flour and was fed to a group of rats.Another group of rat was only fed with corn flour as control.The experiment lastedfor 432 days. 67.6% of the treated animals devloped gross changeson forestomach, such as rough mucosa. disk-like lesions and papillomas. Microsco-pically, 73.5% of the treated animals showed precancerous lesions, while no obser-ved changes were found in the control group.The results show that the residual ofthe Fusarium Poae culture still has tumorigenicity for the forestomach of rat alth-ough the toxin has been extracted from the culture with several solvents.