PCR-SSP检测血小板同种抗原2、3、5系统基因型

Detection of the genotypes of human platelet alloantigen 2,3,5 by PCR sequence specific primers

目的 建立血小板同种抗原 2、3、5系统基因分型方法 ,以检测人群中 HPA-2、3、5系统基因频率。 方法 标本 DNA的抽提采用快速盐析法 ,HPA-2、3、5系统基因分型采用 PCR-SSP方法。 结果 在本研究对象中 HPA-2系统 :a/a基因型频率为 0 .80 6,a/b基因型频率为 0 .1 94 ;a基因频率为 0 .90 3,b基因频率为 0 .0 97。 HPA-3系统 :a/a基因型频率为 0 .4 2 1 ,a/b基因型频率为 0 .5 0 9,b/b基因型频率为 0 .0 70 ;a基因频率为 0 .676,b基因频率为 0 .32 4。HPA-5系统 :a/a基因型频率为 0 .933,a/b基因型频率为 0 .0 67;a基因频率为 0 .967,b基因频率为 0 .0 33。 结论 该方法可鉴定出 HPA-2、3。

Objective In order to establish human platelet alloantigen 2,3,5 systems genotyping methods and detect the allele frequencies of HPA 2,3,5 systems in the population. Methods Genomic DNA is isolated by the quickly salting out method,and the HPA genotyping was detected by PCR sequence specific primers. Results The frequencies of HPA 2a/a homozygote and HPA 2a/b heterzygote were 0.806 and 0.194 in the population,the allele frequencies of HPA 2a and HPA 2b were 0.903 and 0.097. The frequencies of HPA 3a/a homozygote,HPA 3a/b heterzygote and HPA 3b/b homozygote were 0.421,0.509 and 0.070 respectively, the allele frequencies of HPA 3a and HPA 3b were 0.676 and 0.324.The frequencies of HPA 5a/a homozygote and HPA 5a/b heterzygote were 0.933 and 0.067,the allele frequencies of HPA 5a and HPA 5b were 0.967 and 0.033. Conclusion It was indicated that the PCR SSP method can detect the HPA 2,3,5 genotypes.