中国黄牛PrP^c成熟蛋白的原核表达和抗原性分析

Prokaryotic Expression and Antigenic Analysis of PrP^c Mature Protein of Chinese Yellow Cattle

将中国黄牛PrPc 成熟蛋白基因重组质粒b -pET11a -PrPc(本室构建 )转化大肠杆菌BL21(DE3)诱导表达。SDS -PAGE电泳显示表达产物的分子量约为23KD ,大小与预计相符 ;免疫印迹 (WesternBlotting)试验进一步证实 ,中国黄牛PrPc 成熟蛋白获得了正确的表达 ,且与大肠杆菌BL21(DE3)

The recombinant plastid b-pETlla-PrPc of PrPc mature protein gene of Chinese yellow cattle was transformed into E.coi BL21(DE3)for induced expression. SDS-PAGE revealed that the molecular size of the expressed product was about 23KD, and identical to the expected value. West-blotting test confirmed the successful expression of PrPc mature protein of Chinese yellow cattle and it shared same antigen component as E.coli BL 21(DE3).