抗癌药物敏感性的动物试验和临床研究

Basic and Clinical Studies on Assay of Drug Semitivity to Anti— Carcinogens in Nude Mice Bearing Human Tumor

~3H—TdR注入荷瘤裸小鼠腹腔后,0.5h就被增殖旺盛的组织摄取,游离部分大多数在18h内从尿中排出。在抗癌药物作用下,移植瘤、小肠和股骨对~3H—TdR 的摄取量,因其对抗癌药敏感性不同而异。以掺入量为指标,可用于动物模型和临床肿瘤患者个体药敏试验,并可观察其毒性副作用。

The radioactities in tissue samples from nude mice bearing brain human tumor injected with^3H—TdR intraperitoneally were determined with liquid scintillation counter.The results indicatethat the ~3H—TdR is rapidly absorbed by the tissue composed of rapid proliferating cells during the30 min following injection and the remained was discharged from the urine within 18 hours.Un-der the treatment of different anticarcinogens,the ~3H—TdR uptake of xenografts,small intestineand bone marrow were various.It is belived that various amount of uptake is associated with different sensitivities to different anticancer agents.We have employed the method in the experimental model animals and brain tumor cases for studing on the new screening assay of anticarcino-gens using nude mice.