清栓酶对离体大鼠工作心脏缺血再灌注损伤的作用

The Effect of Qingshuanmei on Ischemia-Reperfution Injury in Isolated Working Hearts of rat

离体大鼠工作心脏停灌40min 后再灌注25min,心脏功能明显降低。灌流液中加入清栓酶后能降低室颤发生率;具有明显扩冠作用,增强心肌收缩力,防止LVSP 的下降,促进(?)dp/dt_(max)的恢复。清栓酶对心脏停灌再灌注损伤具有明显保护作用。

An isolated working rat heart underwent normothemic ceasing perfusion for 40 min and reperfusion for 25 min,Qingshuanmei(a viper antithrombotic enzyme)was addedto the perfusate 15 min before ceasing perfusion and 15 min after reperfusion.Theresults indicated that Qinshuanmei(0.625u/L)decreased the incidence of VFsignificantly and improved the contractile function of heart.Qinshuanmei(0.625u/Land 0.938u/L)increased the coronary flow.At the same time,it prevented the decreaseof LVSP and promoted the recovery of +dP/dt_(max)at the concentration of 0.938u/L and0.625u/L Qinshuanmei respectively.We conclude that Qinshuanmei has a benificialeffect to ischemic reperfusion injury in isolated working heart of rat.