乳腺癌血管生成与nm23基因表达及腋窝淋巴结转移的关系

Angiogenesis and the expression of nm23-H_1 tumor metastatic suppressor gene in primary breast carcinoma and their relations to lymph node metastasis

目的 研究乳腺癌血管生成与转移抑制基因nm2 3 H1的表达在肿瘤转移中的作用 ,分析它们与腋窝淋巴结转移的关系。 方法 对 80例乳腺癌患者采用能量多普勒检测肿瘤内血流信号并通过图像分析技术定量测定肿瘤内血管阳性反应总面积 ,对手术切除标本采用免疫组织化学技术检测nm2 3基因蛋白及肿瘤内微血管密度 (microvesseldensity,MVD)的表达。 结果 伴有腋窝淋巴结转移组 (lymphnodepostive ,LN +)肿瘤血管阳性反应总面积、MVD值明显高于不伴有腋窝淋巴结转移组 (lymphnodenegative ,LN - ) (t=7 0 7、6 34,P <0 0 1) ,而nm2 3 H1的表达则相反 ,同时nm2 3 H1阴性表达组的血管阳性反应总面积、MVD值显著高于nm2 3 H1阳性表达组 ,(t =6 86、6 4 9,P <0 0 5 )。 结论 肿瘤血管生成和nm2 3 H1的表达在乳腺癌腋窝淋巴结转移过程中发挥着重要作用。nm2 3 H1基因表达与乳腺癌血管生成可能存在某种内在联系。

Objective To study angiogenesis and the expression of nm23 H 1 tumor metastatic suppressor gene in primary breast carcinoma and their relationship with axillary lymph node metastasis. Methods Intratumoral vascularization in 80 cases of breast cancer was observed preoperatively by power doppler imaging (PDI) and analysed with computer assisted quantative assessment, and the expression of microvessel density (MVD) and nm23 protein was determined by immunohistochemical technique. Results Blood flow signals and blood vessels postitive area within masses were more in axillary node positive (LN+) group than in axillary node negative (LN-) group ( t =7 07, P <0 01), MVD expression were higher in the LN+ group than in the LN- group ( t =6 34, P <0 01). Meanwhile the expression of nm23 H 1 protein was lower in the LN+ group than in the LN- group, and significant correlation was found between angiogenesis and the expression of nm23 H 1 protein. Conclusions Angiogenesis and the expression of nm23 H 1 protein may play an important role in the lymphatic metastasis process of breast cancer. Furthermore, angiogenesis may correlates with the expression of nm23 H 1 protein in that progress.