川芎不同入药形式的体外溶出与药效学比较

Comparison on dissolution rates in vitro and pharmacodynamics of Rhizoma Chuanxiong by different forms used as intermediate

目的 :通过比较川芎超微粉、细粉及水提物、醇提物和双提物的体外阿魏酸溶出量及镇痛作用。方法 :采用桨法体外溶出实验、薄层扫描法测定川芎各入药形式物中阿魏酸的 6 0min体外溶出量 ,用扭体法及热板法比较其镇痛作用。结果 :各入药形式物 (3g)中阿魏酸的溶出量分别为超微粉 (0 .6 45± 0 .0 0 8)mg ,细粉 (0 .35 9± 0 .0 0 5 )mg ,水提物 (0 .2 2 0± 0 .0 0 4)mg ,醇提物 (0 .5 2 6± 0 .0 0 7)mg ,双提物 (0 .0 6 1± 0 .0 0 3)mg ;川芎超微粉的镇痛效果最好。 结论 :川芎超微粉较细粉更能增加有效成分的溶出 ,提高药效。

Objective: To compare the dissolution quantities in vitro of ferulic acid and the analgesic effect of Rhizoma Chuanxiong by supper-micronized powder, fine powder, water extraction, alcohol extraction and double extraction. Methods: Their dissolution rates in vitro were determined by paddle stirring method, the dissolution quantities of ferulic acid in 60 min were determined by TLC-scaning, the analgesic effects were determined by torsion and hot-plate methods. Results: The dissolution quantities of ferulic acid were respectively: supper-micronized powder (0.645±0.008)mg, fine powder (0.359±0.005)mg, water extraction (0.220±0.004)mg, alcohol extraction (0.526±0.007)mg, double extraction (0.061±0.003)mg. The analgesic effect of supper-micronized powder of Rhizoma Chuanxiong was the best. Conclusion: The supper-micronized powder of Chuanxiong improved the dissolution quantity of active components and improved the potency.