麻黄及黄连生物碱膜提取方法的研究

Study on membrane extraction method for alkaloids of Ephedra Herba and Rhizoma Coptidis

目的 :从麻黄和黄连水提液中提取分离生物碱。方法 :采用改性PVA作为分离膜分离生物碱。结果 :提取时间小于8h麻黄草水提液膜分离后为一无色溶液 ,经纸层析实验表明该溶液几乎为纯净的麻黄碱溶液 ,提取时间 8h的分离液是一个 3组分溶液 ;黄连水提液有类似结果。结论 :致密膜分离得到的麻黄、黄连中生物碱纯度高于醇沉方法但生物碱量不及醇沉法。

Objective: Extracting ephedrine and berberine from the water extract of Ephedra Herba and Rhizoma Coptidis. Methods: Modified PVA was used as separating membrane and separated alkaloids. Results: A colorless ephedrine aqueous solution was obtained when extraction time was less than 8 hours; while a three constitution solution was obtained when time of extraction is over 8 hours. So is Rhizoma Coptidis. The membrane is hardly fouled. Conclusion: Purity of alkaloids from Herba Ephedra and Rhizoma Coptidis by membrane separation is Superior to that by alcoholic sediment but amount of alkaloids is reverse.