柴胡与五味子配伍对实验性肝纤维化作用的观察

Observation of action of Bupleurum chinense compability with Schisandra chinensis on experimental hepatic fibrosis in rat

目的 :观察柴胡与五味子配伍提取物对大鼠肝纤维化的作用及机制。方法 :采用皮下注射 40 0ml·L-1CCl4 植物油溶液所致大鼠肝纤维化 ,酶法测定血清中ALT、AST、MAO、ALP、γ GT、TG与 10 0g·L-1肝匀浆MAO、ALP、GSH、TG含量 ,放免法测定血清与肝匀浆中PC Ⅰ、PC Ⅲ水平 ,组织病理学切片检查肝细胞变性程度及胶原纤维增生程度。结果 :柴胡与五味子提取物能显著降低肝纤维化大鼠血清ALT、AST、TG、MAO ,升高血清GSH ,对血清γ GT无影响 ;并且降低肝纤维化大鼠肝匀浆MAO、ALP、PC Ⅰ、PC Ⅲ ,使肝匀浆TG显著升高 ;病理组织检查结果显示 ,柴胡与五味子提取物能使受损肝细胞趋向恢复 ,肝细胞再生功能加强。结论 :柴胡 +五味子提取物对受损或处于受损的肝细胞有保护和修复作用 ,并可消除淤胆 ,阻止肝细胞脂肪性变。

Objective: To investigate the action and mechanism of Bupleurum chinense SD and Schisandra chinensis Turcz extract on liver fibrosis in rat.Methods: The model was prepared by injection of 40% CCl 4 plant oil solution, then the enzyme active AST、AST、MAO、ALP、r-GT、TG in serum and MAO、ALP、GSH、TG in hemogeneous were measured by enzyme method, the level of Pc-Ⅰand PcⅢ were measured by radio-immunity. Results: Bupleurum chinense and Schisandia chinensis extract can reduce significantly enzyme active of ALT、AST、TG、MAO in serum of fibrosis rat, the GSH was elevated and the r-GT was not effect in serum, but it could reduce the level of MAO、ALP、Pc-Ⅰ、Pc-Ⅲ in the homogeneous, sometime the TG was increased and the GSH was not effect in homogeneous. The histopathology showed that Bupleurum chinense and Schisandia chineusis extract could get damaged liver cell back and reinforce the regeneration of hepatic cell. Conclusion:Bupleurum chinense and Schisandid chinensis extract the action of protective and restoration on injured liver cell, and its could eliminate the bile accumulation, to retard the fatty degeneration in fibrotie rat.