HPLC法测定吡拉西坦注射液的含量

Determination of Piracetam Injection by HPLC

目的:建立用高效液相色谱测定吡拉西坦注射液的方法。方法:用Diamonsil C_(18)柱,以甲醇—水(10:90)为流动相,流速0.6ml·min^(-1),检测波长230nm。结果:吡拉西坦线性范围0.17～0.78mg·ml^(-1),r=0.9995。平均回收率为100.0％,RSD为0.24％(n=6)。结论:方法简便、准确、重现性好,可作为该制剂的含量测定方法。

Objective: To establish a determining method of piracetam injection by HPLC. Method: Diamonsil C\g column was used, the mobile phase was methanol-water (10:90) with 0.6 ml·min-1 of flow rate, and the detecting wavelength was at 230 nm. Result: The linear range of piracetam concentration was from 0.17 to 0.78 mg·ml-1 and correlation coefficient was 0. 999 5. The average recevery of sample was 100.0% and RSD was 0.2% (n=6). Conclusion:The method is simple, accurate and a good reproducible. It can be used for determination of piracetam injection.