骨巨细胞瘤中骨保护因子及破骨细胞分化因子的表达

Gene expression of osteoprotegerin and osteoclast differentiation factor in giant cell tumor

目的 检测骨巨细胞瘤中骨保护因子 (OPG)及破骨细胞分化因子 (ODF)mRNA的表达 ,并探讨多核巨细胞的来源及造成骨吸收的分子机制。方法 采用半定量逆转录 聚合酶链反应(RT PCR)方法检测骨巨细胞瘤和正常骨组织中OPG、ODF及其辅助因子巨噬细胞克隆刺激因子、信号受体RANKmRNA的表达 ,并将两种组织中各因子的表达情况进行半定量比较。结果 正常骨组织中OPG、巨噬细胞克隆刺激因子、RANKmRNA有表达 ,ODF呈微弱表达 ;骨巨细胞瘤中OPG、ODF、巨噬细胞克隆刺激因子、RANKmRNA均有表达 ,ODF的表达非常丰富。骨巨细胞瘤中ODF与OPG的比值远远高于正常骨组织。结论 骨巨细胞瘤中的微环境具备破骨细胞形成及其促进骨吸收的必要条件 ,多核巨细胞的来源及其骨吸收功能与上述几种因子的表达有关。

Objective To investigate the gene expression of osteoprotegerin (OPG) and osteoclast differentiation factor (ODF/TRANCE/RANKL), two new members of the TNF-receptor superfamily, in giant cell tumor (GCT); to discuss the molecular mechanism of extensive bone resorption caused by GCT. Methods Using TRIzol reagent to prepare total RNA from GCT sample and normal bone tissue. By a first-strand complementary DNA (cDNA) synthesis kit, cDNA was synthesized from 2.0 μg RNA according to the manufacturer′s instructions. cDNA was then amplified by PCR . Amplification products were resolved by electrophoresis on a 1.5% agarose gel and stained with EB. The relative quantity of the PCR products were determined and the mRNA levels of OPG, ODF, M-CSF (cofactor of ODF), and RANK (receptor of ODF) were compared with that of the normal bone. Results GCT contained highly expressed mRNA of ODF , OPG, M-CSF and RANK. There was mRNA expression of OPG , M-CSF and RANK and less expression of ODF in normal bone. The ODF mRNA and RANK mRNA in GCT were more abundant than that in normal bone. In GCT, the ratio of ODF mRNA exceeded OPG expression. But in normal bone, the OPG mRNA exceeded ODF expression. Conclusions The results suggest that GCT contains all signals including OPG, ODF, M-CSF and RANK that are essential for inducing osteoclastogenesis and promoting bone resorption.