深低温保存家兔股动脉的活力检测

Viability Examination Of Cryopreserved Rabbit Femoral Artery

目的从细胞代谢角度评价深低温保存家兔股动脉的效果。方法动脉材料来自新鲜纯种家兔,分为新鲜组、慢冻组、速冻组共计3组,均进行四唑盐还原试验和氧耗量检测。结果氧耗量测定慢冻组的活力百分率达到实际应用效果,经统计分析慢冻组效果好于速冻组:四唑盐还原试验经统计分析慢冻组好于速冻组,两种方法结果一致。结论活力检测结果表明慢冻组贮存6个月仍有较好活力,慢冻组活力优于速冻组。

Objective To study the effect of Cryop-reserved rabbit femoral artery through metabolic activity.Methods The samples are taken from slaughted rabbits and divided into three groups:fresh group,slow-freezed group,rapid-freezed group.All are observed by using tetrazolum test and oxy-gen consumption examination.Results The ex-amination of oxygen consumption:The Viability of slow -freezed group can be used practically,whose effect is better than that of rapid -freezed group through statistical analysis,The tetrazolium examination effect of slow-freezed group is better than that of the rapid-freezed group.The result of two method is identical.Conclusion The result of viability examination showes that the slow -freezed group perserved with six -months still has better viability,the viability of slow-freezed group is better than that of rapid-freezed group