血管内皮生长因子、碱性成纤维细胞生长因子在非小细胞肺癌中的表达及临床意义

Expression of Vascular Endothelial Growth Factor and Basic Fibroblast Growth Factor in Human Non-Small Cell Lung Cancer and Their Clinical Significance

目的:探讨血管内皮生长因子(VEGF)、碱性成纤维细胞生长因子(bFGF)在非小细胞肺癌(Non-smallcelllungcancer,NSCLC)中的表达及临床意义。方法:应用免疫组织化学方法检测96例NSCLC组织中VEGF、bFGF蛋白水平的表达,其中36例应用RT-PCR技术检测上述基因mRNA表达。结果:NSCLC中VEGFmRNA主要表达分泌性VEGF121和VEGF165,阳性率分别为69.5%(25/36)和41.7%(15/36),bFGFmRNA阳性率为52.8%(19/36);免疫组化显示VEGF、bFGF阳性表达率分别为55.6%(20/36)和58.3%(21/36),二者之间呈正相关(P=0.002);VEGF、bFGF表达与患者年龄、性别、病理类型、分化程度、TNM分期、肿瘤转移、生存期等临床资料之间无统计学意义。结论:VEGF、bFGF在NSCLC血管形成中起重要作用,且二者具有协同作用。

Objective:To explore the expression of vascular endothelial growth factor(VEGF)and basic fibroblast growth factor(bFGF)and their clinical significance in non-small cell lung cancer(NSCLC).Methods:Expression of VEGF and bFGF were examined at the protein levels by immunohistochemical staining in96cases of NSCLC patients,36of them were mea-sured at the mRNA levels by reverse transcription-PCR analysis.Results:VEGF mRNAs were expressed pre dominately as transcripts for the secretory forms of VEGFs (VEGF121and VEGF165)in re sected NSCLC tissues.The positive ratios of VEGF121and VEGF165were69.5%(25/36)and41.7%(15/36)respectively.The positive ratio of bFGF was52.8%(19/36)in the same tumor specimens.The positive ratios of VEGF and bFGF expression protein levels were55.6%(20/36)and58.3%(21/36)respectively.A significant positive correlation was observed between VEGF and bFGF expression in NSCLC(P=0.002).VEGF,bFGF expression and clinical data(age,sex,his to-logical subtype,differentiation,P-stage,metastasis and survival)revealed no signifi cant interre la-tion ship.Conclusion:Our results suggest thast VEGF and bFGF may play an important role in angiogenesis and act in a synergistic manner in NSCLC.