摘要
目的 :研究人类端粒酶RNA(hTR)的反义核酸对Hep 2细胞端粒酶活性的影响。方法 :采用TRAP法检测Hep 2细胞在反义核酸处理前后端粒酶活性的变化。结果 :hTR反义核酸能有效抑制Hep 2细胞的端粒酶的活性。结论 :hTR反义核酸能显著抑制肿瘤细胞的端粒酶活性 。
Objective: To study the imhibitory effect of human telomerase activity by an antisense oligonucleotide against human telomerase RNA(hTR RNA) in Hep 2 cells.Methods: Telomerase activity of Hep 2 cells with antisense treatment was measured using the TRAP assay.Results: The telomerase activity was effectively inhibited by the antisense treatment.Conclusion: Antisense oligonucleotide against hTR can effectively inhibit the telomerase activity of tumor cells and subject is an effective and novel approach for treatment of malignant cancers.
出处
《河南医学研究》
CAS
2002年第1期22-23,共2页
Henan Medical Research
基金
河南省科学技术厅科技攻关项目资助课题 (0 0 1170 2 0 4)
