大鼠脑RC3基因的cDNA克隆及序列分析

The cDNA Cloning and Sequencing of Rat Brain RC3

目的 :为研究脑特异性基因 -RC3的功能 ,克隆该基因的cDNA。方法 :以大鼠脑为材料提取总RNA并分离纯化poly(A)+RNA ,用作模板以嵌套式逆转录PCR方法克隆RC3cDNA ,并将其重组入pUC18质粒 ,然后用双脱氧末端终止法进行碱基序列分析。结果 :大鼠脑RC3cDNA序列有一个碱基差别 ,但不涉及氨基酸的改变。结论 :为进一步研究RC3的功能提供了实验基础。

Objective: To clone the cDNA or a rat RC3 gene,a brain-specific gene. Methods:RC3 cDNA was amplified in the channel of nested RT-PCR with the poly(A)+RNA isolated from the rat brain,and RC3 cDNA fragment was inserted in to pUC18 vector and sequenced by the dideoxy chain termination method.Results:It shows that only one base is different from those reported by Sato-T,which does not involve in change of amino acid.Conclusion:The rat RC3 cDNA obtained basis for futher researching on RC3 function.