大鼠轻度闭合性颅脑损伤后的氧化应激损伤及病理学改变

Experimental study on oxidizing stress injury and pathology changes in rats following an experimental closed traumatic brain injury

目的 :建立清醒状态下大鼠轻度闭合性颅脑损伤模型 ,观察致伤后氧自由基、血糖及病理改变。方法 :用枪击法建立大鼠轻度闭合性颅脑损伤模型 ,采用生化指标脂质过氧化物丙二醛 (MDA)含量和超氧化物歧化酶 (SOD)活力评价其氧化应激损伤 ;用微量法测定血糖水平 ,光镜下观察脑组织形态学改变。结果 :伤后6h脑组织及血清MDA含量均显著升高 (P<0.05) ;血清MDA含量24h达高峰 ,1周后恢复正常 ;伤后脑组织、血清SOD活力及血糖无明显变化 ;光镜下可见脑组织水肿、充血及出血等改变。结论 :轻度闭合性颅脑损伤可导致氧化应激损伤及脑组织病理学改变。

Objective:To study the changes of free radical oxidizing injury,glycaemia and pathology following experimental closed traumatic brain injury in rats.Methods: The brain impact injury was produced by toy gun. The free radical oxidizing injury was evaluated by the content of MDA (Malonic dialdehyde) and the activity of SOD (Superoxide dismutase),the changes of blood glucose level were estimated by microanalysis and the changes of pathology were observed by light microscope.Results: The content of MDA in brain tissue and serum was markedly increased 6h after injury and MDA in serum reached peak at the time of 24h,recovered to normal level 1 week later;there are no changes in pathology of brain, the activity of SOD in brain and serum,and blood glucose level.The pathological edema, congestion and bleeding in brain could be observed by light microscope. Conclusion: The closed traumatic brain injury may induce free oxidizing injury on brain.