S-100蛋白染色和免疫酶组化技术在早期麻风诊断中的作用

Study on S-100 protein immunostaining and peroxidase technique in the early diagnosis of leprosy

目的 : 应用S- 10 0蛋白免疫组化染色 ,探讨其在早期麻风诊断中的作用。方法 : 将 4 6例临床诊断为少菌型麻风 ,病理检查为非特异性炎症 ,未发现神经枝或神经枝结构正常的石蜡包埋块 ,重新作连续切片进行HE染色与S - 10 0蛋白免疫组化染色 ,进行平行对比观察。结果 : 有 11例( 2 3.9% )显示神经内外炎性浸润。 4例 ( 8.7% )发现神经肉芽肿 ,两种方法具有显著性差异 (P <0 .0 5 )。结论 : S 10 0蛋白免疫组化染色在显示神经枝及其病变程度方面优于HE染色 ,有助于早期麻风的诊断 。

Objective: To evaluate the value of S-100 protein immunostaining in the early diagnosis of leprosy.Method: Biopsy specimens from 46 paucibacillary (PB) leprosy patients with nonspecific inflammation, no acid fast bacilli and normal never structure were sliced successively and stained with HE stain and immuno peroxidase of S-100 protein stain. Results: Peripheral never inflammatory infiltration was found in 11 cases and granuloma formation was found in 4 cases with S 100 protein staining. Conclusion : The results shows that S 100 protein staining method play an important role in identification of never damage when the specimen is negative with HE staining. The S 100 protein staining is a very useful method in diagnosis of leprosy, Especially for PB cases.