辐射所致残存染色体易位与放射敏感性关系研究

The Study on the Relationship of Residual Chromosome Aberration Induced by Irradiation with Radiosensitivity

目的 :应用荧光原位杂交 (FISH)方法研究人肿瘤细胞放射敏感性与染色体残存易位关系及临床应用的可行性。方法 :采用 3种放射敏感性不同的人肿瘤细胞系 :鼻咽鳞癌 (CNE)、肺腺癌 (SPC)和乳腺腺癌 (MCF 7)。通过克隆形成方法测定 2Gy、4Gy、6Gy和 8GyX线照射剂量下肿瘤细胞的存活率。采用常规染色体制片过程和 2号染色体涂染探针及FISH方法 ,测定 2Gy、4Gy和 6GyX线照射2 4h后 ,肿瘤细胞 2号染色体内在和诱导的畸变量。结果 :未照射的对照细胞 2号染色体存在不同程度的内在畸变。 2Gy、4Gy和 6Gy照射后 2 4h ,CNE、SPC和MCF 7细胞诱导生成的残存染色体畸变与剂量关系一致 ,能够反映细胞的放射敏感性 ,所有细胞系诱导 2号染色体生成的畸变与细胞存活率均存在良好相关性 (rs=0 96)。结论 :诱导的残存染色体畸变与照射剂量呈线性关系 ,采用FISH方法计数照射诱导的残存染色体畸变 。

Objctive To investigate the relationship of chromosome induced aberration with radiosensitivity of human tumor cells with fluorescence in situ hybridization,exploring its clinical applying possibility.MethodsThree human cell lines of different radiosensitivity:nasopharyngeal cell line(CNE),lung adenocarcinoma cellline(SPC)and breast adenocarcinoma cell line(MCF 7) were used and assessed for clonogenic cell survival after radiation with a range of doses from 2 Gy,4 Gy,6 Gy and 8 Gy and for induced aberation in single chromosome(number2) 24 h after irradiated from 2 Gy,4 Gy and 6 Gy with fluorescence in situ hybridization.Results The number of chromosome 2 induced breaks remaining 24 h after irradiation reflected the radiosensitivity fo the cells such that the relationship between residual chromosome induced aberrations and cell survival was the similar for all cell lines of CNE?SPC and MCF 7 and also have good relationship( r s =0 96).Conclusion These results suggest that the scoring of chromosome induced aberrations in interphase using FISH holds considerable promise for predicting the radiosensitivity of tumor cells.