汉防己甲素联合屈洛昔芬逆转K562/A02细胞耐药的研究

Reversal effect of tetrandrine in combination with droloxifene on cell line K562/A02 and the correlation with apoptosis induction

目的 :探讨汉防己甲素 (Tet)联合屈洛昔芬 (Drol)对耐药细胞系K5 62 /A0 2的逆转作用。方法 :采用四甲基偶氮唑盐比色法 (MTT法 )测定柔红霉素 (DNR)的细胞毒性。结果 :1μmol·L-1Tet、5 μmol·L-1Drol均能增加DNR对耐药细胞系K5 62 /A0 2的细胞毒作用 ,半数抑制量 (IC50 )分别为 (7.2 8± 2 .0 6)mg·L-1和 (7.5 8± 3 .44 )mg·L-1,逆转倍数分别为 2 .94和2 .82倍。两药联合应用后作用明显增强 ,IC50 为 (1.66± 0 .41)mg·L-1,逆转倍数达 12 .9倍。结论 :单独应用Tet、Drol可部分逆转K5 62 /A0 2细胞的耐药性 ,两药联用具有明显协同效应。

Objective To explore the reversal effect of tetrandrine(Tet) in combination with droloxifene(Drol) on multidrug resistant cell line K562/A02.Method The cytotoxicities of daunorubicin(DNR) were assayed by 3(4,5?dimethylthiazole?2?yl)?2,5?diphenyl tetrazolium bromide(MTT) method.Results The cytotoxicities of DNR to K562/A02 were enhanced by 1?μmol·L -1 Tet or 5?μmol·L -1 Drol.Their IC 50 was (7.28±2.06)mg·L -1 and(7.58±3.44) mg·L -1 ,respectively.Their reversal effect on K562/A02 was found to be 2.94 and 2.82 times,respectively.But IC 50 of combined Tet and Drol was (1.66±0.41) mg·L -1 ;its reverse effect increased up to 12.9 times.Conclusion Multidrug resistance(MDR) may be partially reversed by Tet or Drol,but the combination of Tet and Drol shows a greater synergistic reversal interaction.