单细胞凝胶电泳法检测焦炉作业工人淋巴细胞DNA损伤

IYMPHCYTIC DNA DAMAGE OF COKING FURNACE WORKERS WERE DETECTED BY USING SINGLE CELL GEL-ELETROPHORESIS.

目的 :利用单细胞凝胶电泳技术 ,检测焦炉作业工人外周血淋巴细胞 DNA的损伤 ,以了解作业工人所受的遗传损害。方法 :分别抽取炼焦和非炼焦工人进行流行病学调查 ,同时采用单细胞凝胶电泳技术 ,对每一位对象观察 DNA断裂分级 (将 DNA断裂损伤按其损伤程度分级 )。结果 :焦炉作业工人的 DNA断裂程度随生产环境的不同而不同 ,其炉顶、炉侧组均明显高于对照组 ,差异有显著性 (P<0 .0 1) ;专业工龄 10～年的工人 DNA损伤程度远高于专业工龄 0～年及 5～年者 (P<0 .0 1) ;吸烟与 DNA损伤呈正相关 ,而饮酒与 DNA损伤程度未见相关性。结论 :单细胞凝胶电泳法能够快速、敏感地检测炼焦工人淋巴细胞的 DNA损伤 。

Objective:In order to study the hereditary damage of coking furnace workers,iymphcytic DNA damage of peripheral blood were detected by using single cell gel-eletrophoresis.Methods:Epidemiology was studied both on coking furnace workers and none coking furnace worker' peripheral blood.Using single cell gel-eletrophoresis,DNA damage of each sample was classificated.Results:DNA damage degree of coking furnace worker was different following their working environment.The groups at top and lateral part of furnace,DNA damage was significantly higher(P<0.01) than control group.In group who worked more than 10 years,the DNA damage was significantly higher(P<0.01) than in group who worked ≤5 years and worked between 5 and 10 years.There was positive relationship between somking and DNA damage.But,there were no relationship between drinking and DNA damage.Conclusion:Iymphcytic DNA damage could be quickly and sensitively detected by using single cell gel-electrophoresis.