巯基乙酸经呼吸道染毒对小鼠免疫功能的影响

Effect of thioglycollic acid exposure via respiratory tract on the immunological function of mice

目的 研究巯基乙酸经呼吸道染毒对小鼠免疫功能的影响。方法 选择 1 8～ 2 2g昆明种小鼠 ,1 0只 /组 ,雌雄各半 ,以巯基乙酸 0 4,0 8,4 0mg/L经呼吸道静式吸入染毒 ,7天后进行体液免疫、细胞免疫、非特异性免疫及免疫器官脏器系数测试。结果 实验组脾脏的脏器系数均明显低于对照组 (P <0 0 1 ) ,抗体形成细胞 (plague formingcell,PFC)数、巨噬细胞吞噬百分数及吞噬指数均低于对照组 (P <0 0 5,P <0 0 1 )。结论 巯基乙酸经呼吸道吸入染毒对小鼠的体液免疫和非特异性免疫功能可能有抑制作用。

Objective To study the effect of thioglycollic acid exposure by respiratory tract on the immunological function of mice.Method Kunming inbred mice(BW.18～22 gram)were divided into 4 groups,each group had 10 mice(equal in male and female).3 test groups were respectively exposed to 0 4 mg/L,0 8 mg/L and 4 0 mg/L of thioglycollic acid by respiratory tract 2 hours a day for 7days.7 days later,the humoral immunity,cellular immunity,nonspecific immunity and the immune organ weight ratio were all detected.Result The weight ratio of spleen was obviously lower than control(P<0 01),plague forming cell(PFC)and the engulf percentage and engulf index of macrophages were all lower than control(P<0 05 and <0 01).Conclusion Thioglycollic acid exposure by respiratory tract might have some inhibit effect on the humoral immunity and nonspecific immunity of mice.