摘要
分光光度法是一种快速灵敏，操作简便的测定中华芦荟中活性多糖的方法．对于不同种芦荟，其活性多糖的标准曲线不同．取样２．０ｍＬ，加刚果红溶液０．５ｍＬ，浓度为２×１０－４ｍｏｌ／Ｌ，缓冲液酸碱度范围是ｐＨ在８～１４之间，室温下反应时间约２０ｍｉｎ，进行测定，可用于芦荟制品活性成分测定及其产品质量的评价．
The colorimetric assay method is a simple, effective, and sensitive method for quantitatively determining the presence of the bioactive polysaccharide in aloe of China.The best conditions for quantitative determination of a bioactive polysaccharide are 2.0mL of the sample solution and about 0.5mL of 2×10-4mol/L of Congo Red added; the pH's range is from 8 to 14, more preferably from 8 to 11;the reaction time is about 20 minutes at room temperature.
出处
《昆明理工大学学报（理工版）》
2002年第2期21-23,共3页
Journal of Kunming University of Science and Technology(Natural Science Edition)
