GUS基因在杜氏盐藻细胞中的瞬间表达

Transient Expression of GUS Gene in Dunaliella Salina

利用电激法将GUS基因转入杜氏盐藻细胞内进行瞬间表达 ,研究了盐藻的生长状态和电激转化条件对转化率的影响 ,并比较了CaMV35S、Ubil、Ubil Ω、CaMV35S U bil和CaMV35S Ubil Ω 5种启动子的转化效率。结果表明 ,培养 5d的盐藻在 6kV的脉冲电压、0 .0 5s的脉冲持续时间和 2 10的脉冲次数下电激可获得较高的转化率 ,为转化最佳条件。 5种启动子中 ,Ubil Ω启动子的转化效率最高 。

Transient expression of GUS gene in Dunaliella salina was reported by using electroporation. The effects of different growing stages and transfer conditions were studied, the transfer efficiencies of CaMV35S?Ubil?Ubil Ω?CaMV35S Ubil and CaMV35S Ubil Ω promoters were compared. The results showed that higher GUS expression can be gained when cells growing 5 days after inoculation are electroporated under 6 kV pulse voltage?0 05s pulse duration and 210 total number of pulses. Among 5 promoters, Ubil Ω promoter was found to be the most efficient and is suitable for expressing foreign genes in D. salina.