重组鲈鱼生长激素的酶联免疫测定方法

An Indirect Enzyme-linked Immunosorbent Assay for the Recombinant Japanese Set Perch Growth Hormone

建立了重组鲈鱼生长激素 (jspGH)的间接ELISA ,以 jspGH的小鼠多克隆抗体作为一抗 ,HRP羊抗鼠IgG作为二抗 ,在抗体及酶标二抗的稀释液中加入 4 %PEG ,从而提高检测灵敏度至 4 0ng/ml,抗原浓度在 4 0～ 10 0 0ng/ml范围内与OD4 50nm 值成线性关系。此法较简便、灵敏 ,可用于检测基因工程菌发酵液中的jspGH含量。

An indirect enzyme linked immunoassay (ELISA) for recombinant Japanese sea perch growth hormone (jspGH) was established by adding 4%PEG in the first and the second antibody solution. The sensitivity of ELISA is about 40ng/ml. A linear response between the concentration of jspGH and OD 450nm is obtained in the range of 40 1000ng/ml. When the yeast cell line GS115 not containing jspGH was set as the control, the concerntration of jspGH could be calculated from the curve. The method is simple and rapid, and can be used to detect the level of jspGH in fermentaton liquid.