转基因超微量注射控制器的研制及其特性分析

DEVELOPMENT OF TRANSGENIC MICROINJECTION CONTROLLER AND ANALYSIS OF ITS CHARACTERISTICS

注射法是当今构建转基因动物模型的主要方法。注射量是关系到细胞的外源基因容受量及至细胞成活率的重要因素。目前，生物工程界普遍应用的手工注射控制器不能精确控制注射量，注射效率和成活率低下。介绍了一种气压电控式超微量注射控制器的原理与设计。在理论模型推导的基础上，应用多元变换回归设计对出流量与注射时间、注射压力和端内径间的关系进行了正交试验与分析。

Microinjection is a key method to animal gene transfer. Ejection pressure, ejection duration, tip diameter of the pipette are important factors that affect the dispensed volume of the ultramicroinjector. The injection controllers currently used in this field can't accurately control the dispensed volume and thus lead to the low injection efficiency and survival rate. A kind of electrical controller microinjection controller with air as the pressure medium is described. Based on the derivation of theoretical model the relationship between dispensed volume and ejection pressure, ejection duration and tip inner diameter is experimented and analyzed applying multiple factor transformation regression design. The experiment results show that the calibrated microinjection controller can satisfy the requirements of gene transfer.