八正合剂对家兔尿量和离体输尿管平滑肌舒缩功能的影响

Effects of Bazheng Mixture on Urinary Amount and Contractile-Relaxant Function of Isolated Urethral Smooth Muscle in Rabbits

目的 :探讨八正合剂利尿通淋的药理学作用机制。方法 :分别采用导尿管集尿法和离体输尿管环实验 ,观察八正合剂对清醒状态下家兔尿量的影响及家兔离体输尿管平滑肌舒缩功能的影响。结果 :灌胃给药 5 0 g/kg和 10 0 g/kg剂量的八正合剂 ,可使家兔 6 0min尿量显著增加 (P <0 0 5 )。浓度为 9 9～90 9g/L的八正合剂 ,可使家兔离体输尿管环最大舒张力和最大收缩力显著降低 (P <0 0 5 ,P <0 0 1) ;浓度为 2 9 1～ 90 9g/L时 ,离体输尿管环舒缩频率则显著增加 (P <0 0 5 ,P <0 0 1) ;而浓度为 5 6 6 g/L和90 9g/L时 ,离体输尿管环舒缩力振幅也显著增加 (P <0 0 5 ,P <0 0 1) ;在 9 9～ 90 9g/L范围内 ,随着八正合剂浓度的增加 ,离体输尿管环各指标变化率的大小依次为 :振幅 >频率 >最大舒张力 >最大收缩力。结论 :八正合剂的通淋利尿机理与其对输尿管管腔的扩张作用和增强输尿管推进性蠕动作用有关 。

Objective: To explore the pharmacological mechanism of Bazheng Mixture (BZM) in promoting diuresis and relieving stranguria. Methods: By means of urine collection through indwelling catheter and urethral ring isolation test to observe the effects of BZM on the urinary amount of conscious rabbits and contractile relaxant function of urethral smooth muscle isolated from rabbits. Results: BZM, at dosage of 5.0 g/kg or 10.0 g/kg by gastrogavage, could increase the urinary amount in 60 min obviously (P<0 05). For the isolated urethral rings, the maximal contractile and maximal relaxant forces reduced significantly (P<0 05 or P<0 01) when the concentration of BZM applied ranged between 9.9～90.9 g/L; the frequency increased significantly (P<0 05 or P<0 01) when the concentration ranged 29.1～90.9 g/L and the amplitude increased significantly when the concentration were 56.6 and 90.0 g/L. Along with the concentration of BZM increased from 9.9 g/L to 90.9 g/L, the changes occurred in the above mentioned parameters of urethral ring were in order of potency amplitude >frequency > maximal relaxant force > maximal contractile force. Conclusion: The effects of BZM in enhancing urethral peristalsis may be stronger than that in dilating the urethral caliber. Its mechanism in promoting diuresis and relieving stranguria may be related with its action of urethral dilatation and potential peristalsis promotion.