卵巢上皮性肿瘤患者血清与腹水层粘连蛋白的检测及临床意义

Detection of Laminin in Serum and Ascites from Patients with Epithelial Ovarian Tumor and Clinical Implication

研究卵巢上皮性肿瘤患者血清与腹水层粘连蛋白 (L am inin,L N)含量变化及临床意义。采用放射免疫法测定 6 9例卵巢上皮性肿瘤患者及 42例对照组术前及术后血清 L N含量 ,并测定其中 2 2例恶性肿瘤腹水 L N含量。结果显示恶性卵巢上皮性肿瘤组术前血清 L N含量显著高于对照组及良性肿瘤组 (P<0 .0 1) ,术后明显下降 ,手术前后血清L N含量差异有显著性意义 (P<0 .0 5 ) ;卵巢恶性肿瘤患者低度分化组血清 L N含量显著高于中度及高度分化组 (P<0 .0 5 ) ,腹水 L N含量显著高于血清含量 (P<0 .0 5 ) ,淋巴结转移组血清 L N含量显著高于无淋巴结转移组 (P<0 .0 5 )。提示 L N与肿瘤的生物学特性密切相关 。

The change of the laminin (LN) levels of serum and ascites from patients with epithelial ovarian tumor and its clinical implication were studied. Serum and ascites samples from 69 patients with epithelial ovarian tumor and 42 cases as control group were analyzed by radioimmunoassay. The results showed that the LN level (157.85 ±14.37 μg/L)of serum in the patients with malignant tumor was significantly higher than in the control group (125.14 ±7.03 μg/L) and in the patients with benign tumor (128.36 ±8.75 μg/L)(P<0.01)before operation. The serum LN levels in the malignant group were decreased significantly after operation. The serum levels of LN in low-differentiated tumor were higher than in moderate-differentiated tumor and high-differentiated tumor(P<0.05). The LN levels of ascites(172.94±15.26 μg/L) was higher than those of serum (161.34±6.59 μg/L)(P<0.05)in malignant tumor. The serum levels in the patients with lymph node metastasis(165.41±19.91 μg/L)was higher than those without lymph node metastasis(152.35±10.34 μg/L) (P<0.05). It was concluded that LN in serum and ascites expressed higher in malignant epithelial ovarian tumor than in benign tumor. This investigation revealed that LN is one of important diameters reflecting tumor biological characteristics.