摘要
将从钝顶螺旋藻提取、分离的生物活性蛋白 (藻蓝蛋白及藻总蛋白 )与已活化羧基的 4-叠氮苯甲酸反应 ,合成具有光反应性的蛋白质。将蛋白质涂覆在组织培养聚苯乙烯基板 (PSt)上 ,通过紫外光照射 ,蛋白质与聚苯乙烯基板通过共价键连接。研究两种蛋白质接枝生物材料对体外肝癌细胞 740 2生长的抑制作用。
The bioactive protein-phycocyanic and all the proteins of spirulina platensis were isolated and purified. Photo-reactive proteins were synthesized by coupling the proteins with (N-(4-azidobenzoyloxy)succinimide) and were spread onto the 24-well cell culture polystyrene plates. The coated surface was exposed to ultraviolet irradiation for chemical fixation of proteins via the conversion of the phenylazido group to the highly reactive phenyl-nitrene which spontaneously formed covalent bonds with neighboring hydrocarbons. On these proteins-immobilized polystyrene plates, the liver cancer cells 7402 were cultured under the serum-free conditions, and the inhibition activity on proliferation of the liver cancer cells was investigated and analyzed.
出处
《生物医学工程学杂志》
EI
CAS
CSCD
2002年第1期1-3,共3页
Journal of Biomedical Engineering
基金
广东省自然科学基金支持项目 ( 970 848)
