衰减全反射红外光谱用于对牛血清白蛋白在聚醚氨酯改性材料表面吸附的定量分析

Quantitative Characterization of Adsorbed Bovine Serum Albumin on Modified Surfaces of Poly(ether urethane) Materials Using ATR-FT-IR Spectroscopy

根据傅立叶变换红外光谱测试法 (FT- IR)的基本原理 ,利用衰减全反射傅立叶变换红外光谱 (ATR-FT- IR) ,建立了对材料表面蛋白质吸附行为的定量分析方法。并且 ,采用该方法对牛血清白蛋白 (BSA)在聚醚氨酯(PEU)涂覆改性材料表面的静态吸附进行了定量表征。所使用的两种表面改性剂 (SMA)分别是三嵌段偶联物 :十八烷基聚氧乙烯— 4,4'-二苯甲烷二异氰酸酯—十八烷基聚氧乙烯 (简称 MSPEO) ;及另外一种 Cibacron BlueF3G- A端基的同类三嵌段偶联物 (简称 ciba MPEO)。BSA表面吸附实验由静态等温吸附与静态吸附动力学两部分组成。研究结果表明 ,BSA于 MSPEO及 ciba MPEO改性基材表面吸附量明显增加 ,从而确证了这两种 SMA对于BSA的特异吸附作用。

Attenuated total reflection (ATR) FT IR spectroscopy was used to quantitatively characterize the extent of bovine serum albumin (BSA) adsorbed on the surface coating modified poly(ether urethane) (PEU) matrix. The two surface modifying additives (SMA) were respectively a tri block coupling polymer of stearyl poly(ethylene oxide) 4,4′ methylene diphenyl diisocyanate stearyl poly(ethylene oxide), for short MSPEO, and another similar block coupling polymer with the Cibacron Blue F3G A endgroups, for short cibaMPEO. The experiments of static BSA adsorption were composed by two parts. One was static isothermal adsorption, and the other was static adsorption kinetics. The quantitative characterization was based on the optical principles of FT IR, method of experiment and index of the apparatus, by which the enhancement of BSA adsorption on the SMA modified surfaces was confirmed.