一种用于应变场三维细胞培养的组织工程支架

A Tissue-Engineered Strain Scaffold for Three-Dimensional Cell Cultures

我们从组织工程化组织构建的角度 ,提出了一种可用于应变场细胞三维培养的组织工程支架。采用表面化学和材料力学方法 ,探讨了支架的组成、结构、表面特性、力学性质及细胞相容性。利用生物医用聚乙烯醇(PVA)耐水泡沫 ,表面裱衬生物可降解聚乳酸羟基乙酸共聚物 (PL GA)后 ,具有良好的表面特性和适宜的孔隙率 ,既能产生一定的弹性回缩 ,又具有良好的细胞相容性。结果表明 ,PVA耐水泡沫表面裱衬 PL GA为组织工程研究应变对细胞三维培养的影响提供了一种良好的三维支架。

This article introduces a three dimensional scaffold which is used to perform three dimensional cell culture under mechanical stretch from the point of construction of tissue engineered tissue. The composition, structure, surface characteristic, mechanical property, and cell compatibility of the scaffold have been studied by using surface chemistry and material mechanics testing methods. The results indicate that the polyvinyl alcohol (PVA) sponge, which is water tolerant, coated with Poly DL lactic co glycolic acid (PLGA) possesses a good nature in appropriate surface feature, porosity, elastic recoil, and cell compatibility. These features provide wide options for using this scaffold to study the effects of mechanical stretch on cells maintained in three dimensional culture to provide a three dimensional matrix.