定位基因芯片探针点的圆心度方法

Location of the Probe Dots in Gene Chip Image With the Medialness Function

基因芯片扫描图像中探针点的定位是芯片信息提取的基础 ,定位准确性与否直接关系到探针点信息提取的正确性。本文提出一种利用圆心度函数定位基因芯片扫描图像中探针点位置的方法 ,圆心度函数是一种利用边缘信息来检测已知半径圆形区域的有效方法 ,不仅可以突出圆形区域的圆心位置 ,而且抗噪声能力强。基于圆心度分布图和芯片的空间结构 ,本文提出了定位探针点的具体方法 ,实际芯片探针点定位实验验证了本方法的有效性。

For acquisition of the gene chip information, how to correctly locate the probe dots in the chip's scanning image is the base of the chip information processing. Here we present a new approach for locating the probe dots in the gene chip image. First, a medialness function, which is good at detection of circle area with radius given in advance, is used for calculating the medialness map in which the center of circle sample area of the gene chip image is disclosed prominently. Then, a method to locate the probe dots center is given based on the medialness map and the 2D space configuration of the probe dots. The experiments show that the new approach correctly locates the probe dots while against noise affection robustly.