阿诺宁的抗瘤作用研究

Antitumor Effect of Anuoning

背景与目的:我们先前的研究证明,阿诺宁(anuoning)、bullatacin和squamocin在体外有强抗肿瘤作用,squamocin可诱导HL-60细胞凋亡。本研究目的是进一步研究阿诺宁的细胞毒作用和体内抗瘤作用。方法:体外试验用噻唑蓝还原法(MTT法)检测阿诺宁对体外培养的人结肠癌细胞(HT-29)、人鼻咽癌细胞(SUNE1和CNE2)、人肝癌细胞(bel-7402)、人乳腺癌细胞(MCF-7)和人肺腺癌细胞(GLC-82)的生长抑制作用。建立小鼠移植瘤模型,观察阿诺宁腹腔注射对小鼠肝癌(HepS)和肉瘤S-180的体内抗瘤作用。结果:体外实验表明,阿诺宁对CNE2、bel-7402、HT-29和SUNE1细胞的半数抑制浓度(IC50)依次为0.044μg/ml、0.068μg/ml、0.446μg/ml和1.617μg/ml;对MCF-7和GLC-82细胞的IC50分别为1.857μg/ml和3.481μg/ml。体内试验表明,阿诺宁15、30和60μg/kg,腹腔注射作用10天后,对小鼠肝癌的平均抑瘤率依次为36.9%、51.8%和57.9%,与对照组相比有统计学意义;对小鼠肉瘤S-180的平均抑瘤率依次为43.0%、52.1%和61.0%,与对照组相比有统计学意义。结论:阿诺宁对多种人肿瘤细胞有很强的抑制生长作用,并对小鼠移植瘤具有抗瘤作用。

Background &Objective:The authorsprevious studies have demonstrated that anuoning bullatacin and squamocin have anticancer activity in vitro. Squamocin could induce apoptosis of HL 60 cells. The purpose of this paper was to investigate cytotoxicity and antitumor effect of anuoning. Methods:MTT assay was used to examine the growth inhibition of anuoning on human colon carcinoma cell line (HT 29), human nasopharyngeal carcinoma cell line (SUNE1, CNE2), human liver carcinoma (bel 7402), human breast adenocarcinoma cell line (MCF 7) and human lung adenocarcinoma cell line (GLC 82). The models of mice S 180 sarcoma and HepS were used for in vivo antitumor test. Results:The IC50 of anuoning on CNE2, bel 7402, HT 29, SUNE1 cell were 0 044, 0 068, 0 446, and 1 617 μg/ml, respectively. The IC50 of anuoning on MCF 7 cell and GLC 82 cell were 1 857 and 3 481 μg/ml, respectively. Under the doses of 15, 30, and 60 μg/kg, ip, qd×10d, the average tumor inhibitory rates of anuoning to mice tumor HepS were 36 9%, 51 8%, and 57 9%,respectively (P< 0 05). Under the same concentration, the average tumor inhibitory rates of anuoning on mice S 180 sarcoma were 43 0%,52 1%,and 61 0%, respectively (P< 0 05).Conclusions:The results indicate that the anuoning possess cell growth inhibition activity to various human tumor cells in vitro and antitumor effect in vivo.