香菇多糖对巨噬细胞一氧化氮生成和细胞内还原型谷胱甘肽的影响

Effects of lentinan on nitric oxide production and intracellular glutathione in mouse peritoneal macrophages

目的 为探讨香菇多糖 (lentinan,L TN)激活小鼠腹腔巨噬细胞一氧化氮 (NO)生成的作用机制。方法观察 L TN对小鼠腹腔巨噬细胞 NO生成与细胞内还原型谷胱甘肽 (GSH)的影响及其关系。结果 (1) L TN对小鼠腹腔巨噬细胞 NO生成具有明显的促进作用 ,同时细胞内 GSH浓度随 NO生成增加而减少 ;(2 ) NO生成抑制剂能抑制 L TN刺激的小鼠腹腔巨噬细胞 NO生成和细胞内 GSH水平降低 ;(3) GSH去除剂能抑制巨噬细胞NO生成。结论 研究表明 L TN促进小鼠腹腔巨噬细胞 NO生成并同时消耗细胞内 GSH,细胞内 GSH水平可能与 NO的生成有密切关系。提示细胞内 GSH可能起到调节巨噬细胞 NO生成和保护宿主细胞免受

Object To investigate the mechanisms of action of lentinan (LTN) activated mouse peritoneal macrophages to produce nitric oxide (NO). Methods The effects of LTN on NO output and intracellular glutathione (GSH) in mouse peritoneal macrophages and the correlation of them were studied. Results (1) LTN can significantly increase production of NO in mouse peritoneal macrophages, decrease level of intracellular GSH followed increase of NO production. (2) The above effect can be blocked efficiently by the NO production inhibitors. (3) NO production can be inhibited by GSH lowering drugs. Conclusion LTN increase NO production with depletion of intracellular GSH in the activated mouse peritoneal macrophages. It suggested that intracellular GSH plays an important role in regulation of NO production and protection of host cells from cytotoxic attack induced by NO.