经保存血清滴眼液细胞因子、维生素A浓度的稳定性及病原学观察

The stability of cytokines and vitamin A in preserved autologous serum eyedrops and pathogen investigation

目的 探讨不同保存期自体血清滴眼液细胞因子及维生素A(VitA)浓度的稳定性和病原体生长情况。 方法 分别于抽血当时 ,4℃冰箱保存1、4、8周 ,-36℃冰柜中保存4、8周时检测自体血清的表皮生长因子、转化生长因子α、转化生长因子 β 1、胰岛素生长因子Ⅱ、VitA浓度。同时将20 %浓度的自体血清在4°C冰箱及-36℃冰柜保存8周后 ,进行细菌和真菌培养。结果 不同保存温度和保存期血清各种细胞因子和VitA浓度差别均无显著性意义 (均P>0.05)。细菌及真菌培养结果均为阴性。结论

Objective To evaluate the stability of cytokines and vitamin A(VitA) in preserved autologous serum eyedrops and to investigate the possible pathogens. Methods The concentration of EGF,TGF-α,TGF-β1,IGF-Ⅱand VitA in autologous serum eyedrops were measured at the beginning and 1 week,4 weeks,8 weeks after preservation in the refrigerator at 4℃ and after 4 weeks,8 weeks of preservation in the freezer at -36℃.Bacterial and fungus cultures of the 20% concentration of autologous serum were performed.Results The concentration of cytokines and VitA in preserved serum did not significantly change after different preservation period and different preservation temperature (P>0.05).None of the samples was culture positive for bacteria or fungi.Conclusion The concentration of cytokines and VitA in autologous serum eyedrops is stable after long preservation period.