慢性宫颈炎活检组织中人巨细胞病毒(HCMV)DNA和C—MYC癌基因的检测及相关性研究

DETECTION AND CORRELATION OF HUMAN CYTOMEGALOVIRUS DNA AND C--MYC ONCOOGENE IN CHRONIC CERVICITIS

宫颈癌是妇科常见的恶性肿癌之一,而慢性宫颈炎是宫颈癌的高危因素。研究表明,人巨细胞病毒(Human cytomegalovirus,HCMV)感染与宫颈癌的发生有关。并已从宫颈癌组织中鉴定出了C-myc癌基因。为了探讨HCMV感染与C-myc癌基因表达的关系,本文应用核酸杂交技术对87例慢性宫颈炎患者的宫颈活检组织进行了HCMVDNA序列和C-myc癌基因序列的检测。结果87例慢性宫颈炎患者宫颈活检组织中HCMV检出率为11％(10/87),C-myc癌基因的检出率为12％(11/87)。HCMV DNA序列阳性标本中同时C-myc癌基因阳性者有5例(5/10),77例HCMV DNA序列阳性患者中,有6例(6/77),C-myc癌基因阳性。结果提示,HCMV感染对C-myc癌基因表达可能起活化作用。

Cervical caner is one of the most common malignant tumors in gynecopa- thy. Chronic cervicitis is the highly risky factor of cervical cancer. The data indicated that cervical cancer was related to the infection of human cytomegalovirus (HCMV) . C-myc oncogene was detected in the tissues of cervical cancer. In this report, the sequences of HCMV DNA and C- myc oncogene were detected in 87 cases of chronic cervicitis by dot blot hybridization using HCMV DNA and C-myc oncogene probes. The results showed that the positive rates were 11% (10/87) and 12% (11/87), respectively. Both HCMV DNA and C-myc oncogene were identi- fied among the HCMV DNA positive samples in about 50% (5/10), HCMV DNA negtive but C-myc positive spamples were about 7. 88% (6/77). The results showed that HCMV might have activative effect on the expression of C-myc oncogene.