血管紧张素II对单核细胞趋化因子及粘附分子表达的影响

Influences of Angiotensin II on the Expressions of Monocyte Chemokine and Adhesion Molecules

为了观察血管紧张素II (AII)对人单核细胞株THP 1分泌单核细胞趋化因子 (MCP 1)、人类脐静脉内皮细胞(HUVEC )分泌细胞间粘附分子 (ICAM 1)的影响及AII对HUVEC与THP 1的粘附功能的影响 ,我们利用ELISA法检测AII作用后THP 1分泌MCP 1及HUVEC分泌ICAM 1的表达 ,利用计数法观察经AII作用的HUVEC与THP 1的粘附率。结果发现四种不同浓度的AII(10 6、 10 7、 10 8、 10 9mol/L )刺激THP 1及HUVEC 2 4h后 ,MCP 1及ICAM 1的分泌明显增加 ,AII(10 6mol/L )作用HUVEC 6h后 ,其对THP 1细胞粘附率明显增加 ,表明AII具有调节THP 1分泌MCP 1及HUVEC分泌ICAM 1的作用 ,AII可通过致炎症作用参与动脉粥样硬化的发病过程。

To observe the influences of angiotensin II(AII) on the expressions of monocyte chemoattractant protein 1(MCP 1) secreted by human monocyte cell line THP 1 and intercellular adhesion molecule 1(ICAM 1)secreted by human umbilical vein endothelial cell (HUVEC),the effects of AII on the expression of MCP 1 on THP 1 cell and that of ICAM 1 on HUVEC cell were investigated by use of the ELISA assays as well as the determinations of adhesion rates between HUVEC and THP 1 cells after treatment with AII by cell counting It was found that all the 4 different kinds of AII concentrations could stimulate the productions of MCP 1 in THP 1 cells and ICAM 1 in HUVEC cells remarkablly 24 hours after the treatment with AII in a dose dependent manner,and the adhesion rates of HUVEC to THP 1 cells also significantly increased after 6 hours treatment with AII It indicates that AII may contribute to the regulatory effect on the secretion of MCP 1 by THP 1 cells and ICAM 1 by HUVEC cells,and participate in the course of atherogenesis by activation of MCP 1 and ICAM 1