PML-RARα及RARα融合蛋白在三硫化二砷诱导的NB4细胞凋亡中的作用研究

Study on the Role of PML-RARα and RARα Fusion Proteins in NB4 cell Apoptosis Induced by Arsenic Trisulfide

为研究PML RARα及RARα融合蛋白在三硫化二砷 (As2 S3 )诱导的人早幼粒细胞白血病细胞系NB4细胞凋亡中的作用 ,本研究应用细胞形态观察、流式细胞术测定及DNA电泳等方法观察三硫化二砷对NB4细胞的诱导凋亡作用 ,用染色体G显带技术、反转录PCR及Western印迹测定这一过程中PML RARα融合基因及其表达产物、野生型RARα蛋白的变化。结果表明 ,三硫化二砷在 0 .5 - 2 μmol/L之间能诱导NB4细胞凋亡 ,在此过程中PML RARα融合基因无明显变化 ,但PML RARα融合蛋白和野生型RARα蛋白明显减少。结论提示PML

In order to study the role of PML RARα fusion gene and its expression product during apoptotic process in NB4 cells induced by arsenic trisulfide(As 2S 3), the apoptotic effects of NB4 cells were observed by cell morphology, flow cytometry and DNA electrophoresis. The change of PML RARα fusion gene and its expression product were also assayed by chromosomal G banding, RT PCR and Western blot. The results showed that arsenic trisulfide induced apoptosis of NB4 cells, during this process, PML RARα fusion gene had no significant changes, but the expression of PML RARα fusion protein and wild type RARα were all reduced. It is concluded that arsenic trisulfide can induce apoptosis of NB4 cells, the degradation of PML RARα fusion protein and wild type RARα may play an important role during apoptotic process.