Ph^+和Ph^-CML病人外周血TCR Vβ T细胞分布特点

The Distribution Feature of TCR VβRepertoire in Peripheral Blood T Cells from Patients with Ph^+ and Ph^- CML

为了解Ph+ 和Ph-慢性粒细胞白血病 (CML)病人外周血TCRVβ亚家族T细胞的分布特点 ,利用RT PCR分别扩增 13例CML病人 (Ph+ b3a2型 5例 ,Ph+ b2a2型 5例 ,Ph-型 3例 )外周血单个核细胞的TCRVβ的2 4个亚家族基因片段 ,了解病人各Vβ亚家族的利用情况。研究结果 :发现与正常人有所不同 ,病人仅存在部分TCRVβ亚家族T细胞 ,Ph+ b3a2型CML表达 4 - 16 (平均 10 .2 )个Vβ亚家族 ,Ph+ b2a2型CML表达 8- 11(平均8.8)个Vβ亚家族 ,而Ph-者表达 5 - 6 (平均 5 .7)个Vβ亚家族。各病人表达的Vβ亚家族分布有所不同 ,在Ph+(b3a2型 )全部病人表达Vβ10和Vβ16 ,其次为Vβ9和Vβ2 2 ;Ph+ (b2a2型 )除与Ph+ (b3a2型 )相同全部病人表达Vβ10和Vβ16外 ,还全部表达Vβ2 4 ,其次为Vβ8;而Ph-者全部表达Vβ2 4 ,其次为Vβ3,Vβ10 ,Vβ13和Vβ2 2。结论 :提示Ph+ 和Ph-CML病人外周血的TCRVβ亚家族T细胞存在倾斜性分布现象 ,不同类型CML病人T细胞的TCRVβ选择性利用有所不同 。

To investigate the T cell distribution characters of TCR?Vβ repertoire in Ph + and Ph - CML. The 24 subfamilies of the TCR?Vβ genes were amplified in peripheral blood T cells from 13 patients with CML (Ph + b3a2, 5 cases; Ph + b2a2, 5 cases; Ph -, 3 cases) by RT PCR, to analyze the usage of Vβ subfamilies in different CML patients. The results showed that the expression pattern of Vβ repertoire was different in normal individuals and in patients with CML which only have part of Vβ subfamily T cells. 4-16 (mean 10.2) Vβ subfamily T cells were detected in the Ph + b3a2 CML, 8-11(mean 8.8) Vβ subfamily T cells in the Ph + b2a2 CML and 5-6 (mean 5.7) in Ph - CML. Moreover, the expression of Vβ subfamily T cells was different among these three types CML.Vβ10 and Vβ16 were detected in the all cases with Ph + b3a2 and Ph + b2a2 CML, whereas Vβ9 and Vβ22 could be found in the most cases with Ph + b3a2 CML or Vβ24 and Vβ8 in Ph + b2a2 CML. In patients with Ph - CML, Vβ24 were detected in all samples, and Vβ9, Vβ10, Vβ13 and Vβ22 were found in the most cases. The results suggest that skew distribution of TCR?Vβ subfamily T cells was existed in peripheral blood of Ph + and Ph - CML patients. The selected usage of TCR?Vβ is different in various types of CML patients. It may relate to difference of CML cells associated antigen and individual special immunity reaction.