G-CSF对供者淋巴细胞分泌IL-2功能的影响及临床意义

Impact on IL-2 Expression of Lymphocytes in Donors after G-CSF Administration and Its Clinical Significance

为了研究异基因骨髓移植供者使用粒细胞集落刺激因子 (granulocytecolony stimulatingfactor,G CSF)对骨髓及外周血淋巴细胞分泌白细胞介素 2 (IL 2 )的影响 ,进一步探讨G CSF可能通过改变细胞因子分泌平衡而减轻急性移植物抗宿主病 (aGVHD)的发生 ,以 7例健康供者为对象 ,在其应用G CSF前后通过胞内细胞因子染色技术及流式细胞术 ,检测淋巴细胞的IL 2表达 ,结合临床患者骨髓移植后aGVHD发生情况 ,与本科室未行G CSF动员的类同病情、年龄及供、受者有可比性 ,且在同一时期内连续进行的 15例骨髓移植病人aGVHD发生情况进行比较。结果表明 ,7例供者经G CSF动员后外周血及骨髓淋巴细胞IL 2表达与未动员前相比明显减少 ,且接受移植的患者未发生Ⅱ度以上aGVHD ,与既往连续进行移植未行G CSF动员的 15例比较 ,aGVHD有降低趋势。上述结果提示 ,经G CSF动员后 ,供者表达IL 2的淋巴细胞明显减少 ,可能与异基因骨髓移植后患者aGVHD发生减低有关。

In this study, to investigate the effect on expression of IL 2 in lymphocytes from bone marrow and peripheral blood of normal donors after they were mobilized by G CSF in allo BMT, 7 normal donors′ bone marrow and peripheral blood were harvested before and after G CSF administration. The separated lymphocytes were measured by FCM after they were stained intracellularly by anti IL 2, and their expression of IL 2 were compared. The degree of aGVHD in patients after bone marrow transplantation was evaluated clinically, and it was compared with the status of aGVHD of 15 patients whose donors didn′t receive G CSF administration in our department, and 2 groups of patients are comparable in age, types of diseases and status of donors. The results showed that the expression of IL 2 in lymphocytes in 7 G CSF mobilized donors decreased significantly after G CSF administration and more severe aGVHD than grade Ⅱ didn′t develop in these recipient patients, and comparing with 15 patients received the bone marrow from donors who didn′t receive G CSF, the incidence of aGVHD decreased. It is suggested that the expression of IL 2 in lymphocytes was influenced by donors′ G CSF administration, and it is likely that thereby reduces the incidence of aGVHD in patients after BMT.