保存人红细胞的新策略—抗氧化剂保养液保存效果的研究

New Tactics of Human Red Blood Cells Stored at 4℃—Protective Effect of Antioxidant Solution on Red Blood Cells Damage

塑料血袋盛义务献血员的静脉血 (含适量保养液 )并置 4℃冰箱保存。在保存前、后不同时间检查各项指标 ,旨在研究抗氧化剂保养液延长人血红细胞在 4℃条件下的保存时间 ,以缓解血液保存供应的压力 ,为输血抢救创造有利条件。实验分 3组 :ACD组 ,GMA组和抗氧化剂 (SOD)组。研究结果表明 ,在 4℃条件下保存 75天后SOD组红细胞血红蛋白回收率为 87.2 % ,血浆血红蛋白 (mg/L)为 193.2 ,P5 0 (mmHg)为 34.0 (正常值为 33.1) ,最大变形指数 (DImax)为 0 .2 4 13,即为正常值的 74 .3% ,外观检查无明显溶血、变色、气泡和凝块。结论提示 ,用抗氧化剂保养液保存红细胞在 4℃条件下可延长到 75天 ,其红细胞血红蛋白和血浆血红蛋白回收率附合国家《血站基本标准》

Aliquots of venous blood from healthy donor were collected in plastic blood storage bags with ACD, GMA or antioxidant solution(superoxide dismutase, SOD), respectively, and stored at 4℃. After storage for varying periods, the parameters of the blood were detected in the blood samples. Results showed that the parameters of the blood stored at 4℃ for 75 days in SOD group were following: the recovery of RBC Hb was 87.2%, plasma Hb(mg/L) was 193.2, P50(mmHg) was 34.0 (normal value was 33.1); deformability(DImax) was 0.2413 (74.3% of normal value). There was no evident hemolysis, color change, air bubble and clots. It was concluded that human RBC stored at 4℃ for 75 days with SOD solution, recovery of levels of RBC Hb and plasma Hb were accorded with the requirements of 《Basic Demands of Blood Station》 in China.