阿司匹林和吲哚美辛对幽门螺杆菌生长的抑制作用

Inhibitory effects of aspirin and indomethacin on the growth of H.pylori in vitro

目的 探讨体外培养条件下 ,阿司匹林和吲哚美辛对幽门螺杆菌 (Hp)生长的影响 ,以及阿司匹林存在时 ,Hp对几种常用抗生素敏感性的影响。 方法 Hp接种于含不同浓度阿司匹林或吲哚美辛的布氏肉汤中培养 ,测定不同培养时间菌液的吸光度 ,稀释法计数活菌数 ,革兰染色观察细菌形态。琼脂稀释法测定阿司匹林和吲哚美辛的最低抑菌浓度 (MIC)。E 检测法检测Hp对甲硝唑、克拉霉素及羟氨苄青霉素的MIC。结果 阿司匹林和吲哚美辛可抑制Hp的生长 ,这种抑制作用呈剂量依赖性 ,与培养基 pH改变无关。 4 0 0 μg/ml阿司匹林或 10 0 μg/ml吲哚美辛体外培养 4 8h可使Hp完全溶解破坏。阿司匹林和吲哚美辛对Hp的体外MIC90 分别为 5 12和 12 8μg/ml。阿司匹林存在时 ,分别使10 0 % ,75 %及 75 %的Hp对羟氨苄青霉素、甲硝唑及克拉霉素的MIC降低 ,提示阿司匹林可使Hp对上述抗生素的敏感性增高。结论 阿司匹林和吲哚美辛可抑制Hp的生长 ,阿司匹林可提高Hp对甲硝唑。

Objective To investigate the possible effects of aspirin and indomethacin on the growth of Helicobacter pylori(H.pylori) and the effects of aspirin on the susceptibility of H.pylori to some antimicrobials. Methods Kinetic studies of H.pylori were performed by inoculating strains in Brucella broth with different concentrations of aspirin and indomethacin. Growth of bacteria in the broth was assessed spectrophotometrically and by viable colony count after incubation for 24 and 48 hours. Bacteria morphology was studied by Gram stain under light microscopy. MICs of aspirin and indomethacin were determined by standard agar dilution method. MICs of amoxicillin, clarithromycin and metronidazole were measured in the presence and in the absence of aspirin by E test method. Results Kinetic studies revealed that aspirin and indomethacin inhibited the growth of H.pylori in a dose dependent manner and the bactericidal activities of these agents were due to cell lysis. Aspirin at 400 μg/ml produced about 2 logs decrease in CFU per milliliter at 48 hours. The assemble inhibition effects were obtained when 100 μg/ml indomethacin tested. The MIC 90 of H.pylori for aspirin and indomethacin were 512 μg/ml and 128 μg/ml, respectively. Increased susceptibility to amoxicillin, clarithromycin and metronidazole of H. pylori were induced by aspirin. Conclusions Aspirin and indomethacin could significantly inhibit the growth of H.pylori when incubated in Brucella broth in vitro. A subinhibitory concentration of aspirin enhanced the susceptibility of H. pylori to antimicrobial agents.