咳停片对豚鼠咳嗽反射的抑制作用及析方研究

The antitussive effect of Keting tablets and analysis of the prescription

目的 :观察咳停片的镇咳作用 ,进行初步析方研究 ,评价该处方的配伍合理性。方法 :分复方组 (咳停片 )、无氯组 (不含氯化铵的咳停片 )和氯化铵组 3组 ,比较 3组对枸橼酸雾化吸入或电刺激喉上神经引起的豚鼠咳嗽反射的抑制作用。结果 :复方组和氯化铵组能抑制枸橼酸引起的豚鼠咳嗽反射 ,复方组的作用强度是氯化铵组的 1.3 2倍 ,是无氯组 4倍以上 ,无氯组无明显作用。复方组、无氯组能抑制电刺激喉上神经引起的豚鼠咳嗽反射 ,复方组的作用强度是无氯组的 2 .94倍 ,氯化铵组无作用。结论 :咳停片处方中剔除氯化铵后镇咳作用减弱 ,表明氯化铵与处方中的中药成分有协同作用 。

OBJECTIVE:The study aimed to observe the antitussive effect of Keting tlabets(KTT) and analyze the prescription to assess its reasonableness.METHOD:The prescription divided into three groups,such as compound prescription group(KTT),non ammonium chloride group(including all the compenents of KTT but ammonium chloride) and ammonium chloride group.Firstly,the cough reflex induced by inhalation of citric acid aerosol was evaluated in conscious guinea pigs.Secondly,the centrally mediated cough reflex induced by afferent electrical stimulation of the superior laryngeal nerve were investigated in anesthetized guinea pigs.RESULTS:Both of KTT and ammonium chloride groups had antitussive effect on guinea pigs that induced by citric acid,the effect potent of KTT group was 1.32 times that of ammonium chloride group and over 4 times that of non ammonium chloride group.Non ammonium chloride group had no significant effects.Meanwhile,both KTT and non ephedrine had antitussive effect on the centrally mediated cough reflex induced by afferent electrical stimulation of the superior laryngeal nerve in anesthetized guinea pigs.The effect potent of KTT group was 2.94 times that of non ammonium chloride group.Ammonium chloride group had no obvious effect.CONCLUSION:The antitussive effect of KTT excluding ammonium chloride was weaker than that of KTT.The results demonstrated that there was a synergism between ammonium chloride and the Chinese medicine of TKN,and the prescription was not only rational but also feasible.