高效毛细管电泳法测定伤痛外搽酊中毒性成分的透皮吸收量

Determination on quantum through skin of toxicanlt Composition in Shangtong Weicha Tincture by HPCE

目的 :观察伤痛外搽酊中毒性成分在体内外透皮吸收强度。方法 :采用高效毛细管电泳法测定了毒性成分在体外的渗透量和动物体内血液中毒性成分的透皮吸收量。结果 :体外法表明士的宁、马钱子碱的平均透过率分别为 :0 .5 63μg/ cm2 · h和0 .5 90μg/ cm2 · h;体内法表明伤痛外搽酊低、高剂量组家兔血液中均未检出乌头碱 ,低剂量组家兔血液中未检出士的宁 ,高剂量组家兔血液中士的宁含量为 3 2 .44 ng/ ml。结论 :本品中士的宁在动物体内外均有极微量的透皮吸收作用 。

OBJECTIVE:To observed intensity of penetration skin absorption of toxicanlt composition in Shangtong Weicha Tincture.METHOD:The osmotic quantum of toxicant composition in blood outside animal bodies and inside animal bodies was determinated by HPCE.RESULTS:In vitro test indicated that the average penetration rate of strychnine and brucine are 0.563μg/cm 2·h and 0.590μg/cm 2·h. In vivo test indicated that there are not aconitine in the rabbits bloods of low and high dosage. The low dosage are not strychnine. The strychnine in rabbits bloods of the high dosage was penetrated 32.44ng/ml.CONCLUSION:The strychnine in the tincture which was penetrated skin is very microdose. Aconitine was not absorpted by skin of rabbits.