丹参素抑制过亚硝酸根引发的鲁米诺发光和蛋白质酪氨酸硝化

Danshensu inhibit peroxynitrite-induced luminol chemiluminescence and protein tyrosine nitration

目的 :研究在生理 p H条件下丹参素对过亚硝酸根引发的鲁米诺化学发光反应和蛋白质酪氨酸硝化反应的影响 ,以便从分子水平上了解丹参素的药理作用机制。方法 :用发光强度和 43 0 nm处的吸光度分别对鲁米诺发光产物和酪氨酸硝化产物进行定量。结果 :发光反应和硝化反应都剂量依赖地受到丹参素的抑制。丹参素对反应的抑制强于抗坏血酸和半胱氨酸。结论 :丹参素可有效抑制过亚硝酸根引发的反应 。

OBJCTIVE: To study the effect of danshensu on the peroxynitrite induced luminol chemiluminescence and protein tyrosine nitration at physiological pH so as to understand the medicinal mechanism of dashensu on a molecular level. METHODS: The chemiluminescent product of numinol was evaluated with the peak value of light emission and the nitrated product of tyrosine was measured spectrophotometrically at 430nm. RESULT: Danshensu inhibited both the chemiluminescence and the nitration in a concentration dependent manner. The inhibition of dandhensu to the reactions is more effective than ascorbate and cysteine. CONCLUTION: Danshensu can inhibit the peroxynitrite induced reations effectively. This is an important molecular mechanism of pharmacodynamics of dashensu.