复方板蓝根颗粒定量方法的研究

A study on the Quantitative Method of Indigo and lndirubin in Fufang Banlangen Keli

目的 :建立复方板蓝根颗粒中靛蓝和靛玉红的含量测定方法。方法 :采用反相高效液相色谱法 ,固定相为 Spherisorb C1 8柱 ,以醋酸铵 -甲醇 (3 7∶ 63 ,v∶ v)为流动相 ,检测波长 2 80 nm,测定该制剂中靛蓝和靛玉红的含量。结果 :靛蓝平均回收率为99.2 % ,RSD为 1.0 % ;靛玉红平均回收率为 10 1.1% ,RSD为 1.8%。结论 :本法操作简便 ,结果准确 。

OBJEETIVE: To establish an accurate method for the determination of indigo and indirubin in Fufang Banlangen Keli. METHOD: The HPLC method was employed with a spherisorb C 18 column(4.6mm×250mm). 0.1 mol/L ammonium acetate methanol (37∶63, v∶v) was as the mobile phase, The wavelength of detection was 280nm. RESULTS: The mean recoveries for indigo and indirubin were (99.2±1.0)% and (101.1±1.8)%, respectively. CONLUSION: The method was simple, accurate and suitable for assay of indigo and indirubin in the preparation.